Introdução: A tuberculose é considerada uma ameaça global a saúde pública, estando entre as dez doenças que mais geram óbitos. O surgimento de cepas resistentes aos tratamentos convencionais, promoveu um interesse no desenvolvimento de novos agentes terapêuticos. O cobre é o terceiro elemento de transição mais abundante no organismo humano, apresentando baixa citotoxicidade e potencial atividade antimicobacteriana. O complexo Cu(II) com isoniazida avaliado neste estudo ([CuCl2 (INH) 2] H2O - I1) foi encapsulado em matriz polimérica de Eudragit® NE 30D. O Eudragit® é um polímero sintético da família de copolímeros de polimetacrilato, amplamente utilizado para liberação controlada por medicamentos em filmes, revestimentos ou usado como matriz em várias formulações farmacêuticas orais. Objetivo: Desta forma, o objetivo deste estudo foi avaliar o potencial antimicobacteriano, citotóxico e mutagênico da nanopartícula de Eudragit® NE 30D e o complexo I1 (NP-NE30D). Material e Métodos: A avaliação citotóxica foi realizada em duas linhagens celulares, sendo uma fibroblástica não transformante (GM07492) e uma linhagem derivada de hepatocarcinoma humano (HepG2), utilizando-se da substância reveladora resazurina. A atividade mutagência foi executada de acordo com a metodologia de pré-incubação, desenvolvida por Maron & Ames, e a concentração inibitória mínima (MIC90) das amostras frente a cepa padrão de Mycobacterium tuberculosis H37Rv (ATCC 27294) foi determinada pelo ensaio de microtitulação com resazurina (REMA - Resazurin Microtiter Assay). Resultados: A NP-NE30D demonstrou atividade antimicobacteriana moderada, MIC90 191,0 ± 15,1 μmol/L e baixa citotoxicidade com IC50 de 569,6 ± 6,9 e 414,6 ± 19,2 μmol/L, para GM07492 e HepG2, respectivamente. Sendo assim, a NP-NE30D necessita de uma concentração duas vezes maior para HepG2 e três vezes maior para GM07492, para causar a citotoxicidade gerada ao M. tuberculosis. Além disso, não induziu mutagenicidade pelo teste de Ames. Conclusão: O presente estudo contribui para a busca de novas formulações seguras com atividades biológicas, uma vez as propriedades avaliadas podem indicar se o composto é um candidato promissor para o tratamento de tuberculose.
Publicado
2020-07-01
Como Citar
Andrade Aleixo, N. ., Bento da Silva, P. ., Pavan, F. R. ., Castro, G. R. ., & Resende, F. A. . (2020). AVALIAÇÃO DA ATIVIDADE ANTIMICOBACTERIA DE COMPLEXO DE COBRE(II) CONTENDO ISONIAZIDA NANOENCAPSULADO EM MATRIZ POLIMÉRICA. Revista Multidisciplinar Em Saúde, 1(2), 49. Recuperado de http://editoraime.com.br/revistas/rems/article/view/231
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