AVALIAÇÃO DA ATIVIDADE ANTI-INFLAMATÓRIA DA Tribulus terrestris EM RAW 264.7 ESTIMULADA COM LPS

Autores

  • José Vinicius Firmiano Alves Faculdade Presidente Antônio Carlos
  • Victor Fernandes Ribeiro
  • Jair Adriano Kopke Aguiar Faculdade Presidente Antônio Carlos
  • Luiz Gustavo Oliveira Faculdade Presidente Antônio Carlos

Palavras-chave:

RAW 264.7, Tribulus terrestris, Anti-inflamatório

Resumo

Introdução: Tribulus terrestris é uma planta rastejante, do gênero Zygophyllaceae, de origem de regiões temperadas e tropicais da Europa, do sul da Ásia, da África, da Austrália e do Sudão. Foram descritas uma diversidade de atividades farmacológicas desta planta, dentre elas podem ser citadas as propriedades afrodisíacas, anti-inflamatórias, antimicrobianas e potenciais antioxidantes. Existem vias de sinalização nas células RAW 264.7, que ativam uma variedade de fatores de transcrição, incluindo NF-kB. Objetivo: Avaliar os efeitos de Tribulus terrestris no processo inflamatório estimulado por LPS em linhagem de macrófagos RAW 264.7. Material e métodos: Como matéria prima foi utilizado extrato seco de Tribulus terrestris, a viabilidade celular da linhagem RAW 264.7 em contato com a Tribulus terrestris foi avaliada por ensaio de MTT com as concentrações (10; 20; 40; 60; 80; 100; 150; 200; 300; 400 e 500 μg/mL). Foi determinada a concentração de óxido nítrico (NO), pelo método de Griess, a Translocação do NF-kB foi realizado pelo teste de imunofluorescência determinando como o NF-kB se manifesta quando a célula entra em contato com a Tribulus terrestris. Resultados: O teste de viabilidade celular apontou para uma IC50 de 226,1 μg/mL. Observamos que em macrófagos estimulados com LPS e tratados com Tribulus terrestris houve redução na concentração de NO, quando comparados com o controle. Além disso, verificamos uma menor translocação de NF-kB quando a célula é tratada pela Tribulus terrestris. Conclusão: Foi demonstrado que a Tribulus terrestris exerce efeitos anti-inflamatórios por reduzir mediadores pró-inflamatórios, inibindo também a translocação de NF-kB.

Publicado

2020-04-01

Como Citar

Firmiano Alves , J. V., Fernandes Ribeiro, V. ., Kopke Aguiar, J. A. ., & Oliveira, L. G. . (2020). AVALIAÇÃO DA ATIVIDADE ANTI-INFLAMATÓRIA DA Tribulus terrestris EM RAW 264.7 ESTIMULADA COM LPS. Revista Multidisciplinar Em Saúde, 1(1), 13. Recuperado de https://editoraime.com.br/revistas/rems/article/view/98

Edição

v. 1 n. 1 (2020): Edição Especial: Anais do I Congresso Brasileiro de Ciências Farmacêuticas On-line

Seção

Anais de Evento

Licença

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

O conteúdo desta revista foi migrado para https://editoraintegrar.com.br

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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
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