ASSOCIAÇÃO DO POLIMORFISMO GENÉTICO DA CROMOGRANINA A (CHGA) E CÂNCER DE TIREOIDE
Autores
Jhon Willatan Saraiva Siqueira
Universidade de Brasília
Bruna Rodrigues Gontijo
Universidade de Brasília
Caroline Ferreira Fratelli
Universidade de Brasília
Rafael Martins de Morais
Universidade de Brasília
Izabel Cristina Rodrigues da Silva
Universidade de Brasília
Palavras-chave:
Polimorfismo Genético, Cromogrania A, Neoplasias da Glândula Tireoide
Resumo
Introdução: A Cromogranina (CHGA) é uma glicoproteína fosforilada, expressa em vários tecidos neuroendócrinos, e seu gene está localizado no cromossomo 14q32. A CHGA tem sido indicado como biomarcador em outras doenças como neurodegenerativas e neuropsiquiátricas, hipertensão, doenças cardiovasculares, e até mesmo em câncer de próstata. Poucos estudos associam alterações na expressão da proteína com outras cânceres. Objetivo: Analisar a relação entre a presença do polimorfismo na região codante do gene CHGA Glu264Asp e a susceptibilidade ao carcinoma papilífero de tireoide (CPT). Material e Métodos: realizou-se um estudo transversal, de caso-controle em 56 participantes (ambos os sexos, sendo 23 no grupo caso). O grupo CPT possuía histórico de tireoidectomia e tratamento com o Iodeto de Sódio (I131). Para o estudo do polimorfismo genético, extraiu-se o DNA de amostras de sangue com o kit minispin blood (MN®), e a genotipagem foi executada com a técnica PCR-RFLP, com a enzima de restrição DpnI (Thermo Fisher, 2h, 37ºC). O alelo A (polimórfico) apresentou dois fragmentos de DNA, com 177pb e 41pb. As análises estatísticas foram executadas com o Teste Qui-quadrado. Adotou-se o nível de significância de 5%. O presente projeto foi aprovado no comitê de ética (CAAE 57382416.6.0000.0023). Resultados: Houve diferença estatística significativa na distribuição alélica entre o grupo CPT e o controle, sendo que o alelo G (ancestral) estava mais frequente no grupo CPT (93,5% contra 78,8% no grupo controle; P = 0,033, OR= 3,86). Semelhante ao presente estudo, a análise efetuada por Ma e seus colaboradores (2010) revelou associação significativa entre o alelo G e o risco de câncer de próstata na população japonesa. Conclusão: O alelo ancestral G pode ser um fator de risco para o desenvolvimento do CPT. No entanto, por ser uma doença de etiologia multifatorial, são necessários outros estudos em populações diferentes para melhor compreensão da doença.
Publicado
2020-04-01
Como Citar
Saraiva Siqueira, J. W. ., Rodrigues Gontijo, B. ., Ferreira Fratelli, C. ., Martins de Morais, R. ., & Rodrigues da Silva, I. C. (2020). ASSOCIAÇÃO DO POLIMORFISMO GENÉTICO DA CROMOGRANINA A (CHGA) E CÂNCER DE TIREOIDE. Revista Multidisciplinar Em Saúde, 1(1), 12. Recuperado de http://editoraime.com.br/revistas/rems/article/view/97
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