ASSOCIAÇÃO DO POLIMORFISMO GENÉTICO DA CROMOGRANINA A (CHGA) E CÂNCER DE TIREOIDE

Autores

  • Jhon Willatan Saraiva Siqueira Universidade de Brasília
  • Bruna Rodrigues Gontijo Universidade de Brasília
  • Caroline Ferreira Fratelli Universidade de Brasília
  • Rafael Martins de Morais Universidade de Brasília
  • Izabel Cristina Rodrigues da Silva Universidade de Brasília

Palavras-chave:

Polimorfismo Genético, Cromogrania A, Neoplasias da Glândula Tireoide

Resumo

Introdução: A Cromogranina (CHGA) é uma glicoproteína fosforilada, expressa em vários tecidos neuroendócrinos, e seu gene está localizado no cromossomo 14q32. A CHGA tem sido indicado como biomarcador em outras doenças como neurodegenerativas e neuropsiquiátricas, hipertensão, doenças cardiovasculares, e até mesmo em câncer de próstata. Poucos estudos associam alterações na expressão da proteína com outras cânceres. Objetivo: Analisar a relação entre a presença do polimorfismo na região codante do gene CHGA Glu264Asp e a susceptibilidade ao carcinoma papilífero de tireoide (CPT). Material e Métodos: realizou-se um estudo transversal, de caso-controle em 56 participantes (ambos os sexos, sendo 23 no grupo caso). O grupo CPT possuía histórico de tireoidectomia e tratamento com o Iodeto de Sódio (I131). Para o estudo do polimorfismo genético, extraiu-se o DNA de amostras de sangue com o kit minispin blood (MN®), e a genotipagem foi executada com a técnica PCR-RFLP, com a enzima de restrição DpnI (Thermo Fisher, 2h, 37ºC). O alelo A (polimórfico) apresentou dois fragmentos de DNA, com 177pb e 41pb. As análises estatísticas foram executadas com o Teste Qui-quadrado. Adotou-se o nível de significância de 5%. O presente projeto foi aprovado no comitê de ética (CAAE 57382416.6.0000.0023). Resultados: Houve diferença estatística significativa na distribuição alélica entre o grupo CPT e o controle, sendo que o alelo G (ancestral) estava mais frequente no grupo CPT (93,5% contra 78,8% no grupo controle; P = 0,033, OR= 3,86). Semelhante ao presente estudo, a análise efetuada por Ma e seus colaboradores (2010) revelou associação significativa entre o alelo G e o risco de câncer de próstata na população japonesa. Conclusão: O alelo ancestral G pode ser um fator de risco para o desenvolvimento do CPT. No entanto, por ser uma doença de etiologia multifatorial, são necessários outros estudos em populações diferentes para melhor compreensão da doença.

Publicado

2020-04-01

Como Citar

Saraiva Siqueira, J. W. ., Rodrigues Gontijo, B. ., Ferreira Fratelli, C. ., Martins de Morais, R. ., & Rodrigues da Silva, I. C. (2020). ASSOCIAÇÃO DO POLIMORFISMO GENÉTICO DA CROMOGRANINA A (CHGA) E CÂNCER DE TIREOIDE. Revista Multidisciplinar Em Saúde, 1(1), 12. Recuperado de http://editoraime.com.br/revistas/rems/article/view/97

Edição

v. 1 n. 1 (2020): Edição Especial: Anais do I Congresso Brasileiro de Ciências Farmacêuticas On-line

Seção

Anais de Evento

Licença

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

O conteúdo desta revista foi migrado para https://editoraintegrar.com.br

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