PERFIL CITOGENÉTICO E MOLECULAR DA LEUCEMIA LINFOBLÁSTICA AGUDA EM CÉLULAS T, DE PACIENTES PEDIÁTRICOS

Autores

  • Emanuela Bezerra Gueiros
  • Maria do Socorro Pombo Oliveira
  • Terezinha de Jesus Marques Salles
  • Elda Pereira Noronha

Palavras-chave:

citogenética, biologia molecular, leucemia

Resumo

Introdução: A LLA-T é uma neoplasia maligna hematológica agressiva e representa 15% das LLAs da infância. Atualmente, são descritas anormalidades genéticas específicas com importância no diagnóstico e prognóstico da doença, sendo assim é de suma importância os estudos citogenéticos e moleculares para a compreensão dessa malignidade e conduta de tratamento. Objetivo: O objetivo deste trabalho foi estudar o perfil genético e molecular dos pacientes pediátricos portadores de LLA-T (0-18 anos) através de marcadores clínicos e biológicos que possam contribuir na identificação de fatores de prognóstico da doença. Material e métodos: Participaram do estudo 138 crianças com LLA T, na qual foram utilizadas técnicas de citogenética clássica, bandeamento G e biologia molecular (RT-PCR) para investigação dos achados genéticos dos pacientes. O estudo foi realizado no Instituto Nacional do Câncer, durante o ano de 2015, com material biológico de pacientes que foram diagnosticados entre 2010 e 2015. O programa de hematologia-oncologia pediátricos do Instituto Nacional do Câncer é referência para leucemia aguda, onde são diagnosticadas e tratadas crianças e adolescentes de diversos estados. Desta forma, tem fundamental importância aprimorar o diagnóstico com introdução de técnicas diagnósticas atualizadas, que permitirão definir melhor as características clínicas e laboratoriais e correlaciona-las com a sobrevida e com os dados da literatura, permitindo uma melhor avaliação dos riscos e perfil dos casos de LLA T no Brasil. Resultados: Dos 138 pacientes estudados a maioria foi do sexo masculino (74%) com média de idade de 10 anos. A análise da fusão SIL-TAL1 foi realizada em 70 amostras que obtiveram material suficiente, na qual 13 foram positivas para essa fusão; No HOX11L2, foram possíveis fazer a análise em 71 amostras, sendo duas delas positivas e no MLL/ENL as análises foram realizadas em 92 amostras, 3 delas positivas. Conclusão: De acordo com os achados laboratoriais e clínicos analisados nesse estudo, os resultados encontraram-se coerente aos já encontrados na literatura, sendo que para uma investigação com resultados mais sólidos, faz-se necessário, mas tempo de estudo, sendo que não foi suficiente para alcançar todos os objetivos propostos no início do projeto.

Publicado

2020-09-01

Como Citar

Gueiros, E. B., Oliveira, M. do S. P., Salles, T. de J. M., & Noronha, E. P. (2020). PERFIL CITOGENÉTICO E MOLECULAR DA LEUCEMIA LINFOBLÁSTICA AGUDA EM CÉLULAS T, DE PACIENTES PEDIÁTRICOS. Revista Multidisciplinar Em Saúde, 1(3), 52. Recuperado de https://editoraime.com.br/revistas/rems/article/view/428

Edição

v. 1 n. 3 (2020): Edição Especial: Anais do I Congresso Brasileiro de Saúde On-line

Seção

Anais de Evento

Licença

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

O conteúdo desta revista foi migrado para https://editoraintegrar.com.br

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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php