DIAGNÓSTICO SIMULTÂNEO DE TUBERCULOSE E COVID-19 EM AMOSTRAS DE ESCARRO

Autores

  • Thayná Rosa Bispo
  • Lucas Ventura de Souza
  • Andréa Gobetti Coelho Bombonate
  • Akemi Oshiro Guirelli
  • Andreia Moreira dos Santos Carmo

DOI:

https://doi.org/10.51161/rems/3260

Palavras-chave:

COVID-19, DIAGNÓSTICO, MYCOBACTERIUM TUBERCULOSIS, SARS-COV-2, TUBERCULOSE

Resumo

Introdução: Desde o início da pandemia de COVID-19, têm sido notificados casos de coinfecção por Complexo Mycobacterium tuberculosis (CMTB) e SARS-CoV-2, o que pode elevar o potencial de morbidade e mortalidade. Tais casos têm importância em saúde pública, sobretudo em populações sob condições de vulnerabilidade. Objetivo: Avaliar a detecção simultânea de SARS-CoV-2 e CMTB em população privada de liberdade (PPL), utilizando amostras de escarro e a plataforma GeneXpert. Material e métodos: Estudo prospectivo da precisão diagnóstica do Teste Rápido Molecular Xpert® Xpress SARS-CoV-2 em amostras de escarro. Para a detecção dos genes E e N2 de SARS-CoV-2 foi utilizado o cartucho Xpert Xpress SARS-CoV-2. Para a detecção dos genes IS6110 e IS1081 do CMTB e o gene de resistência à rifampicina foi utilizado o cartucho Xpert MTB/RIF Ultra. Com coletas paralelas de amostras de secreção da nasofaringe, a identificação da Covid-19 foi comparada com a  metodologia padrão-ouro, RT-qPCR, utilizando o protocolo Charité com a detecção dos genes E, N e RdRP/S. Resultados: Foram testadas 282 amostras, com 13 detecções de CMTB, sem resistência a rifampicina; 14 detecções de SARS-CoV-2 e uma detecção de coinfecção CMTB/SARS-CoV-2. O escarro se mostrou vantajoso quando comparado com a secreção nasofaríngea, uma vez que, além de não causar desconforto ao paciente durante a coleta, não é necessária a atuação de um profissional para a sua execução. Conclusão: O estudo ganha uma inovação pela utilização de escarro na detecção do SARS-CoV-2 com o Teste Rápido Molecular Xpert® Xpress SARS-CoV-2, pois a Food and Drug Administration (FDA) dos EUA emitiu autorização para seu uso em amostras nasofaríngeas ou lavado/ aspirado nasal. O protocolo desenvolvido se mostrou simples e rápido, com desempenho e resultados comparáveis à metodologia padrão-ouro. Ainda, a realização dos dois testes simultâneos para a detecção do CMTB e SARS-CoV-2 em uma única amostra de escarro pode agilizar o atendimento e tratamento dos indivíduos, importante para todos, mas sobretudo para aqueles em condições de vulnerabilidade, como é o caso da população privada de liberdade, suportando as ações de interrupção da cadeia de transmissão de ambas as doenças. 

Publicado

2022-03-16

Como Citar

Bispo, T. R. ., Souza, L. V. de ., Bombonate, A. G. C. ., Guirelli, A. O. ., & Carmo, A. M. dos S. . (2022). DIAGNÓSTICO SIMULTÂNEO DE TUBERCULOSE E COVID-19 EM AMOSTRAS DE ESCARRO. Revista Multidisciplinar Em Saúde, 2(4), 313. https://doi.org/10.51161/rems/3260

Edição

Seção

I Congresso Brasileiro de Saúde Pública On-line: Uma abordagem Multiprofissional
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PKPApplication.execute() % line   68, file: index.php
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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php
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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php

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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php

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                                                         SubmissionSearch._getMergedPhraseResults(Object:Journal, Array[1], , null, null, 500, 1) % line  146, file: SubmissionSearch.inc.php
                                                      SubmissionSearch._getMergedKeywordResults(Object:Journal, Array[4], , null, null, 500, 1) % line  194, file: SubmissionSearch.inc.php
                                                   SubmissionSearch._getMergedPhraseResults(Object:Journal, Array[4], null, null, null, 500, 1) % line  146, file: SubmissionSearch.inc.php
                                                SubmissionSearch._getMergedKeywordResults(Object:Journal, Array[3], null, null, null, 500, 1) % line  132, file: SubmissionSearch.inc.php
                                             SubmissionSearch._getMergedArray(Object:Journal, Array[1], null, null) % line  263, file: SubmissionSearch.inc.php
                                          SubmissionSearch.retrieveResults(Object:Request, Object:Journal, Array[1], null, null, null, Object:DBResultRange, Array[1]) % line   92, file: RecommendBySimilarityPlugin.inc.php
                                       RecommendBySimilarityPlugin.callbackTemplateArticlePageFooter(Templates::Article::Footer::PageFooter, Array[3]) % line  107, file: HookRegistry.inc.php
                                    HookRegistry.call(Templates::Article::Footer::PageFooter, Array[3]) % line 1267, file: PKPTemplateManager.inc.php
                                 PKPTemplateManager.smartyCallHook(Array[1], Object:Smarty_Internal_Template) % line   43, file: 42362804174f266c598cdb32c3208fc745b92101^1c372c95cd85572e0fbc9a53d0323a0b229cfc78_0.app.frontendpagesarticle.tpl.php
                              content_6781613650b432_13896783(Object:Smarty_Internal_Template) % line  123, file: smarty_template_resource_base.php
                           Smarty_Template_Resource_Base.getRenderedTemplateCode(Object:Smarty_Internal_Template) % line  114, file: smarty_template_compiled.php
                        Smarty_Template_Compiled.render(Object:Smarty_Internal_Template) % line  216, file: smarty_internal_template.php
                     Smarty_Internal_Template.render(false, 1) % line  232, file: smarty_internal_templatebase.php
                  Smarty_Internal_TemplateBase._execute(Object:Smarty_Internal_Template, null, 42362804174f266c598cdb32c3208fc745b92101, null, 1) % line  134, file: smarty_internal_templatebase.php
               Smarty_Internal_TemplateBase.display(frontend/pages/article.tpl, null, 42362804174f266c598cdb32c3208fc745b92101, null) % line  924, file: PKPTemplateManager.inc.php
            PKPTemplateManager.display(frontend/pages/article.tpl) % line  292, file: ArticleHandler.inc.php
         ArticleHandler.view(Array[1], Object:Request) % line  391, file: PKPRouter.inc.php
      PKPRouter._authorizeInitializeAndCallRequest(Array[2], Object:Request, Array[1], false) % line  231, file: PKPPageRouter.inc.php
   PKPPageRouter.route(Object:Request) % line  143, file: Dispatcher.inc.php
Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php