ALTERAÇÃO DA HISTOARQUITETURA TESTICULAR EM RATOS WISTAR APÓS ADMINISTRAÇÃO DE 5-FLUOROURACIL

Letícia Ganem Rillo Paz  Barateiro; Pedro Luiz Zonta De  Freitas; Lilian Catarim  Fabiano; Nilza Cristina  Buttow; Jaqueline De Carvalho  Rinaldi

doi:10.51161/rems/1574

ALTERAÇÃO DA HISTOARQUITETURA TESTICULAR EM RATOS WISTAR APÓS ADMINISTRAÇÃO DE 5-FLUOROURACIL

Autores

Letícia Ganem Rillo Paz Barateiro
Pedro Luiz Zonta De Freitas
Lilian Catarim Fabiano
Nilza Cristina Buttow
Jaqueline De Carvalho Rinaldi

DOI:

https://doi.org/10.51161/rems/1574

Palavras-chave:

MORFOLOGIA, QUIMIOTERAPIA, SISTEMA REPRODUTOR MASCULINO, TESTÍCULO

Resumo

Introdução: A quimioterapia com 5-fluorouracil (5-FU) promove diversos efeitos colaterais. Estudos anteriores demonstram que esse quimioterápico altera a morfologia dos túbulos seminíferos e possibilita a descamação do epitélio germinativo do testículo. Entretanto, há poucos dados sobre os impactos do 5-FU nesse órgão em dose e terapêutica semelhantes à prática clínica. Objetivo: Avaliar o impacto da administração de 5-FU na morfologia do testículo de ratos adultos. Material e métodos: O estudo foi aprovado pelo Comitê de Ética no Uso de Animais da Universidade Estadual de Maringá (protocolo: 4422140918). Foram utilizados 14 ratos Wistar machos, divididos em dois grupos (n=7): grupo controle (GC), administrado com solução fisiológica via intraperitoneal; grupo quimioterapia (G5-FU), administrado pela mesma via com 15 mg/kg de 5-FU por quatro dias consecutivos, 6 mg/kg por mais quatro dias alternados e dose final de 15 mg/kg. Esse esquema é semelhante ao utilizado para o tratamento dos cânceres: colorretal, mama, estômago, pâncreas, fígado, útero, ovário e bexiga. No 15° dia, todos os animais foram eutanasiados por superdosagem de Tiopental Sódico. O testículo foi dissecado, fixado em Methacarn e incluído em blocos de parafina. Cortes semi-seriados de 5 μm foram corados com Hematoxilina e Eosina para avaliação morfológica pelo método de Weibel. Por fim, realizou-se a estatística e os valores foram expressos em média (porcentagem) ± erro padrão. Resultados: Por análise comparativa, foi observado alteração significativa nos compartimentos epitelial (GC= 58,94 ± 0,52; G5-FU= 55,37 ± 0,52) e estromal (GC= 31,48 ± 0,60; G5-FU= 34,81 ± 0,63) do testículo após administração de 5-FU. Esses resultados sugerem a descamação epitelial dos túbulos seminíferos frente à quimioterapia. Apesar do modelo experimental diferente, nossos resultados corroboram com outros estudos na literatura. Conclusão: A quimioterapia com 5-FU em posologia semelhante à humana alterou a histoarquitetura testicular de ratos Wistar.

Publicado

2021-07-28

Como Citar

Barateiro, L. G. R. P. ., Freitas, P. L. Z. D., Fabiano, L. C. ., Buttow, N. C. ., & Rinaldi, J. D. C. . (2021). ALTERAÇÃO DA HISTOARQUITETURA TESTICULAR EM RATOS WISTAR APÓS ADMINISTRAÇÃO DE 5-FLUOROURACIL. Revista Multidisciplinar Em Saúde, 2(3), 42. https://doi.org/10.51161/rems/1574

Baixar Citação

Edição

v. 2 n. 3 (2021)

Seção

I Congresso Nacional Multidisciplinar de Oncologia On-line

Licença

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

O conteúdo desta revista foi migrado para https://editoraintegrar.com.br

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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php

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                                                         SubmissionSearch._getMergedPhraseResults(Object:Journal, Array[1], , null, null, 500, 1) % line  146, file: SubmissionSearch.inc.php
                                                      SubmissionSearch._getMergedKeywordResults(Object:Journal, Array[4], , null, null, 500, 1) % line  194, file: SubmissionSearch.inc.php
                                                   SubmissionSearch._getMergedPhraseResults(Object:Journal, Array[4], null, null, null, 500, 1) % line  146, file: SubmissionSearch.inc.php
                                                SubmissionSearch._getMergedKeywordResults(Object:Journal, Array[3], null, null, null, 500, 1) % line  132, file: SubmissionSearch.inc.php
                                             SubmissionSearch._getMergedArray(Object:Journal, Array[1], null, null) % line  263, file: SubmissionSearch.inc.php
                                          SubmissionSearch.retrieveResults(Object:Request, Object:Journal, Array[1], null, null, null, Object:DBResultRange, Array[1]) % line   92, file: RecommendBySimilarityPlugin.inc.php
                                       RecommendBySimilarityPlugin.callbackTemplateArticlePageFooter(Templates::Article::Footer::PageFooter, Array[3]) % line  107, file: HookRegistry.inc.php
                                    HookRegistry.call(Templates::Article::Footer::PageFooter, Array[3]) % line 1267, file: PKPTemplateManager.inc.php
                                 PKPTemplateManager.smartyCallHook(Array[1], Object:Smarty_Internal_Template) % line   43, file: 42362804174f266c598cdb32c3208fc745b92101^1c372c95cd85572e0fbc9a53d0323a0b229cfc78_0.app.frontendpagesarticle.tpl.php
                              content_6781613650b432_13896783(Object:Smarty_Internal_Template) % line  123, file: smarty_template_resource_base.php
                           Smarty_Template_Resource_Base.getRenderedTemplateCode(Object:Smarty_Internal_Template) % line  114, file: smarty_template_compiled.php
                        Smarty_Template_Compiled.render(Object:Smarty_Internal_Template) % line  216, file: smarty_internal_template.php
                     Smarty_Internal_Template.render(false, 1) % line  232, file: smarty_internal_templatebase.php
                  Smarty_Internal_TemplateBase._execute(Object:Smarty_Internal_Template, null, 42362804174f266c598cdb32c3208fc745b92101, null, 1) % line  134, file: smarty_internal_templatebase.php
               Smarty_Internal_TemplateBase.display(frontend/pages/article.tpl, null, 42362804174f266c598cdb32c3208fc745b92101, null) % line  924, file: PKPTemplateManager.inc.php
            PKPTemplateManager.display(frontend/pages/article.tpl) % line  292, file: ArticleHandler.inc.php
         ArticleHandler.view(Array[1], Object:Request) % line  391, file: PKPRouter.inc.php
      PKPRouter._authorizeInitializeAndCallRequest(Array[2], Object:Request, Array[1], false) % line  231, file: PKPPageRouter.inc.php
   PKPPageRouter.route(Object:Request) % line  143, file: Dispatcher.inc.php
Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php

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