PERFIL DE SUSCEPTIBILIDADE E PRODUÇÃO DE BIOFILME DE ESPÉCIES CANDIDA ISOLADAS DE CORRENTE SANGUÍNEA DE NEONATOS CRÍTICOS

Autores

  • Ralciane de Paula Menezes
  • Priscila Guerino Vilela Alves
  • Nagela Bernadelli Sousa Silva
  • Maria Gabriela Ferreira
  • Denise Von Dolinger De Brito Röder

DOI:

https://doi.org/10.51161/rems/1186

Palavras-chave:

ANTIFÚNGICOS, BIOFILME, CANDIDA SPP., NEONATOS

Resumo

Introdução: Espécies de Candida são comensais, porém têm-se observado um aumento na ocorrência de candidemia em neonatos com baixo peso ao nascer, submetidos a procedimentos invasivos e em uso prolongado de antibióticos de amplo espectro. A expressão de alguns fatores de virulência por Candida spp., como a formação de biofilme, é crucial no desenvolvimento e manutenção de infecções e está associado à de resistência aos antifúngicos. Objetivos: Avaliar a susceptibilidade aos antifúngicos e a produção de biofilme de Candida spp. isoladas de corrente sanguínea de neonatos. Material e Métodos: Foram estudados quatros isolados obtidos de neonatos admitidos na Unidade de Terapia Intensiva Neonatal de um hospital universitário, sendo dois de C. albicans, um de C. glabrata e um de C. parapsilosis. A susceptibilidade aos antifúngicos anfotericina B, fluconazol e micafungina foi determinada pelo método de microdiluição em caldo. A capacidade de formação de biofilme foi avaliada em placas de fundo chato de 96 poços através da quantificação da biomassa, utilizando cristal violeta, e da atividade metabólica por meio do ensaio de redução do sal de sódio XTT, e mensuradas em espectrofotômetro. Os isolados foram classificados de acordo com a absorbância em: não produtores (DO<0,44), produtores moderados (0,440,2). Resultados: A CIM50 (concentração que inibiu 50% dos isolados) foi 0,25 mg/mL para o anfotericina B, 1 mg/mL para o fluconazol e 0,03 mg/mL para micafungina e apenas o isolado de C. parapsilosis foi resistente à micafungina. Todos os isolados foram forte produtores de biomassa (OD entre: 0,556 [C. parapsilosis] e 3,836 [C. glabrata]) e apresentaram alta atividade metabólica, com valores de OD entre 0,240 (C. glabrata) e 1,027 (C. albicans). Conclusão: Apenas um isolado apresentou resistência aos antifúngicos testados. No entanto, a maioria apresentou uma intensa capacidade de formação de biofilme, revelando o potencial de virulência dos isolados estudados.

Publicado

2021-06-12

Como Citar

Menezes, R. de P. ., Alves, P. G. V. ., Silva, N. B. S., Ferreira, M. G., & Röder, D. V. D. D. B. (2021). PERFIL DE SUSCEPTIBILIDADE E PRODUÇÃO DE BIOFILME DE ESPÉCIES CANDIDA ISOLADAS DE CORRENTE SANGUÍNEA DE NEONATOS CRÍTICOS. Revista Multidisciplinar Em Saúde, 2(2), 29. https://doi.org/10.51161/rems/1186

Edição

v. 2 n. 2 (2021): Edição Especial: Anais de Eventos

Seção

Anais do I Congresso Nacional de Microbiologia Clínica On-Line

Licença

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

O conteúdo desta revista foi migrado para https://editoraintegrar.com.br

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